Selenium metabolism in rats with long-term ingestion of Se-methylselenocysteine using enriched stable isotopes
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- Tsuji Yoshiro
- Graduate School of Pharmaceutical Sciences, Chiba University
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- Suzuki Noriyuki
- Graduate School of Pharmaceutical Sciences, Chiba University
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- T. Suzuki Kazuo
- Graduate School of Pharmaceutical Sciences, Chiba University
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- Ogra Yasumitsu
- Graduate School of Pharmaceutical Sciences, Chiba University
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抄録
Se-Methylselenocysteine (MeSeCys) is not only a selenium (Se) supplement but also a more promising precursor of an anti-tumor drug containing Se than selenomethionine, which is currently used as Se supplement. In this study, the metabolism of MeSeCys labeled with an Se isotope, 82Se, in rats depleted of endogenous natural abundance isotopes with another Se isotope, 78Se, was traced for 21 days when MeSeCys was continuously and perorally ingested at a supplemental dose. The tracer experiment was performed with our improved method that utilized an inductively coupled plasma-deuterium reaction-mass spectrometer. The substitution of endogenous Se with a single isotope, 78Se, facilitated the detection of exogenous labeled Se. Exogenous Se in the form of MeSeCys preferably accumulated and/or assimilated in the liver, kidneys and testes with long-term ingestion of MeSeCys and was utilized for the synthesis of selenoproteins, i.e., extracellular and cellular glutathione peroxidases and selenoprotein P. Meanwhile, intact MeSeCys was not excreted into urine although trimethylselenonium was detected in addition to selenosugar. The results suggest that MeSeCys was transformed into selenide via methylselenol by β-lyase. Consequently, it is surmised that MeSeCys is a precursor of methylselenol under long-term ingestion.
収録刊行物
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 34 (2), 191-200, 2009
一般社団法人 日本毒性学会
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詳細情報 詳細情報について
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- CRID
- 1390282679877948416
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- NII論文ID
- 110007160540
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- NII書誌ID
- AN00002808
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- ISSN
- 18803989
- 03881350
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- NDL書誌ID
- 10258526
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 使用不可