Chromosome Preparation from the Medaka, Oryzias latipes, at the Different Stages of Development

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The first studies concerning the chromosomes of the medaka, Oryzias latipes, have been done more than 70 years ago by Goodrich (1927). Detailed karyotype of the medaka has been reported by Ojima and Hitotsumachi (1969). Here we introduce easy methods for karyotyping of the medaka both adult fish and embryos on the early stages of development. These methods were developed in our laboratory by modification of the previous ones reported by Arai (1973) and Klegerman and Bloom (1977).

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