2. アスパラガス野外茎頂の凍結保存における前処理の影響

• 実山 豊

  北海道大学農学部

• 鈴木 卓

  北海道大学農学部

• 原田 隆

  北海道大学農学部

• 藤川 清三

  北海道大学低温科学研究所

書誌事項

タイトル別名

• 2. Effects of Pretreatments on Cryopreservation in Asparagus Shoot Apices

抄録

Shoot apices of asparagus (Asparagus officinalis L.) can be successfully cryopreserved (by freezing at a rate of 0.5℃/min in the presence of 8% DMSO and 0.2 M sorbitol) after pretreatments either by precultufe in media containing 0.5 M glucose for 2 days or by cold acclimation at 0℃ for 20 days. In the present study, we examined the effects of pretreatments for subsequent successful cryopreservation of the shoot apices. Freezing tolerance, as revealed by LT_<50> under equilibrium-freezing, was -20℃ in both specimens with pretreatment by 0.5 M glucose and by cold acclimation, while LT_<50> was -7℃ in control specimens. Both pretreatments produced distinct cellular ultrastructural changes as observed with ultra-thin section electron microscopy. Number of plastids rich in starch granules increased in precultured specimens, whereas those poor in the starch granules increased in cold acclimated specimens. Occurrence of freezing injury by equilibrium-freezing, as observed with a freeze-fracture replica technique, was associated with formation of aparticulate domains with accompanying fracture-jump lesions in the plasma membranes, not only in precultured specimens with 0.5 M glucose and in cold acclimated specimens, but also in control specimens.

収録刊行物

• 低温生物工学会誌

  低温生物工学会誌 41 (2), 50-57, 1995

  低温生物工学会

キーワード

• 茎頂
• 凍結保存
• 耐凍性
• 微細構造変化

詳細情報

• CRID: 1390001205086099968
• NII論文ID: 110007367434
• NII書誌ID: AN10448734
• DOI: 10.20585/cryobolcryotechnol.41.2_50
• ISSN: 24241555
• ISSN: 13407902
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可