書誌事項
- タイトル別名
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- 4. Hypothermic Preservation of Cultured Cells using Fish Type III Antifreeze Protein from Notched-fin Eelpout(Papers presented at the 54^<th> Annual Meeting, June 14, 2008, Ishikawa)
- ナガガジ由来3型不凍蛋白質の細胞保護機能に関する研究
- ナガガジ ユライ 3ガタ フトウタンパクシツ ノ サイボウ ホゴ キノウ ニ カンスル ケンキュウ
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Antifreeze protein from Notched-fin eelpout (nfeAFP) has an ability to preserve mammalian cells in hypothermic condition. We compared the preservation ability between the naturally extracted isoform mixtures (denoted nfeAFPs) and a recombinant single isoform (denoted nfeAFP6) through observation of cultivated cells (HepG2) preserved at 4℃ for 24 hr with Euro-Collins solution in the presence of 0 - 10 mg/ml of the AFP samples. The preserved cells were examined by fluorescence microscopy after staining with calcein-AM and propidium iodide and by evaluations of extracellular lactate dehydrogenase (LDH) and intracellular ATP content. It appeared that nfeAFPs preserve the cell viability more effectively than nfeAFP6. In addition, the preservation ability increased dose-dependently and is maximized at 10 mg/ml of the protein concentration. These results suggest that addition of nfeAFP decreases hypothermic stress and the mixture of nfeAFP isoforms is more effective than the single isoform.
収録刊行物
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- 低温生物工学会誌
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低温生物工学会誌 54 (2), 93-96, 2008
低温生物工学会
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詳細情報 詳細情報について
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- CRID
- 1390001205087366016
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- NII論文ID
- 110007367490
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- NII書誌ID
- AN10448734
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- ISSN
- 24241555
- 13407902
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- NDL書誌ID
- 10208210
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可