33.麹菌のジテルペン合成酵素(口頭発表)

DOI

書誌事項

タイトル別名
  • 33. Characterization of diterpene synthase genes in Aspergillus oryzae

抄録

Fungi produce diterpenes exhibiting phytohormone-related activities, such as gibberellin and fusicoccin. We identified a diterpene synthase gene PaFS, which is responsible for fusicoccin biosynthesis, in the mycelium of fusicoccin-producing fungus Phomopsis amygdali (Toyomasu et al., PNAS, 104: 3084-3088, 2007). PaFS (719 amino acids) possesses the diterpene cyclase domain at N-terminus and prenyltransferase domain at C-terminus. Our database search suggested that PaFS homologues exist in genome DNA of several fungal species. Among them, we found two PaFS homologous genes in Aspergillus oryzae genome database. It has been believed that A. oryzae produces few secondary metabolites including terpenoids. In this study, we characterize the two homologues, A. oryzae diterpene synthase like 1(AoDSL1) and AoDSL2. AoDSL1 contained a stop codon in region encoding prenyltransferase domain probably because of naturally occurred nonsense mutation, and is perhaps expressed as a truncated enzyme composed of N-terminal 412 amino acid residues. AoDSL2 encoded 728 amino acid residues, in which a successive Asp of DDxxD motif in the prenyltransferase domain was substituted by AN (position 482-483). These possible naturally occurred mutations perhaps resulted in loss of prenyltransferase activities. Recombinant AoDSL1 converted GGDP into two unknown diterpene cyclic hydrocarbons. The effects of site directed mutagenesis of possible naturally occurred mutation on enzyme activity will be presented.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390001204423054848
  • NII論文ID
    110007468293
  • DOI
    10.18978/jscrpanb.44.0_47
  • ISSN
    21896313
    09191887
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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