83.イネにおけるジテルペノイドフィトアレキシンの生合成遺伝子ノックダウン株の作出(口頭発表)
書誌事項
- タイトル別名
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- 83. Preparation of knockdown plants for diterpenoid phytoalexin biosynthetic genes in rice
抄録
Phytoalexin are low-molecular-weight compounds produced and accumulated after exposure to microorganism attack, elicitor and ultraviolet, and have been suggested to serve as plant antibiotics. Rice (Oryza sativa) produces 14 diterpenoid phytoalexins, including oryzalexins A-F, phytocassanes A-E, oryzalexin S, and momilactones A and B. We have identified 6 diterpene cyclase genes responsible for biosynthesis of these phytoalexins: OsCPS2, 4, OsKSL4, 7, 8, and 10. In this study, we attempted to produce diterpenoid-phytoalexin-deficient rice plants by knock down of OsCPS2/4, OsKSL4, or OsKSL7 to verify the roles of these phytoalexins on disease resistance in rice. We first found one line of rice plant in which Tos17 was inserted in the 3rd intron of OsCPS4 by PCR screening of Tos17 mutant panel. However, the level of the mature OsCPS4 mRNA in the line slightly lower than that in wild type rice, suggesting that Tos17 in the 3rd intron of OsCPS4 was excluded by splicing. This result showed that this line is not a null mutant for OsCPS4. Next, we prepared transgenic rice, in which each RNAi trigger (approximately 100bp) for OsCPS2/4, OsKSL4, and OsKSL7 was expressed under the control of rice ubiquitin promoter. However, specific knock down of the targeted genes were not observed in the transgenic rice plants. We have made again transgenic rice plants in which approximately 200bp trigger sequences were introduced, and will present the transcript levels in the transgenic plants.
収録刊行物
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- 植物化学調節学会 研究発表記録集
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植物化学調節学会 研究発表記録集 44 (0), 97-, 2009
一般社団法人 植物化学調節学会
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詳細情報 詳細情報について
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- CRID
- 1390001204422415232
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- NII論文ID
- 110007468343
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- ISSN
- 21896313
- 09191887
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可