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The purpose of this study was to investigate the influence of physical activity on taste thresholds to obtain the knowledge for preventing voluntary dehydration. Taste thresholds were measured in 18 university student athletes (baseball players) before and after a routine training. Taste responses to sucrose (sweet), NaCl (salty), citric acid (sour) were tested. The values of detection threshold for sour were significantly decreased. The recognition threshold for salty was also significantly decreased. Otherwise recognition threshold for sweet was not significant, but prone to decrease. There was also rise in salivary pH and improvement of mood after exercise. These results indicate that taste thresholds change after physical activity. This variation of taste thresholds could be influenced by sweat loss during exercise. These results suggest that the body biotransformation caused by sweat loss may change taste thresholds after severe physical activity.