Application of waste water (Jomen-sui) from Lentinula edodes sawdust Jomen-culture for laccase production

DOI Web Site 26 References
  • SATO Toshitsugu
    Microorganism Research Department, Iwate Biotechnology Research Center:Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology
  • NAGAI Masaru
    Microorganism Research Department, Iwate Biotechnology Research Center
  • WATANABE Hisayuki
    Microorganism Research Department, Iwate Biotechnology Research Center
  • TAKAHASHI Machiko
    Microorganism Research Department, Iwate Biotechnology Research Center
  • NAKADE Keiko
    Microorganism Research Department, Iwate Biotechnology Research Center
  • SAKAMOTO Yuichi
    Microorganism Research Department, Iwate Biotechnology Research Center
  • YAMAUCHI Takahiro
    Edible Mushrooms Institute, Hokken Co., Ltd.
  • AOKI Takayuki
    Batoh Plant, Hokken Co., Ltd.
  • EDA Katsumasa
    Edible Mushrooms Institute, Hokken Co., Ltd.
  • AYUSAWA Sumio
    Edible Mushrooms Institute, Hokken Co., Ltd.
  • OZAWA Kiichi
    Environment Science Department, Research Institute for Environmental Science and Public Health of Iwate Prefecture
  • YOSHIDA Toshihiro
    Environment Science Department, Research Institute for Environmental Science and Public Health of Iwate Prefecture
  • UCHIMIYA Hirohumi
    Microorganism Research Department, Iwate Biotechnology Research Center

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Other Title
  • シイタケ上面栽培廃液(上面水)のラッカーゼ製剤としての利用
  • シイタケ ジョウメン サイバイ ハイエキ ジョウメンスイ ノ ラッカーゼ セイザイ ト シテ ノ リヨウ

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Abstract

Lentinula edodes (Shiitake mushroom) secretes various enzymes, including laccase (Lcc), which is a lignin degrading enzymes with environmental bioremediation advantages. In this study, we investigated enzyme activity in the waste water ("Jomen-sui") from L. edodes sawdust Jomen-culture, removal of endocrine disrupting chemicals (EDCs) by Jomen-sui, changes of Lcc activity in Jomen-sui, and the concentration methods of Jomen-sui. Lcc, cellulase, xylanase, and acid proteinase activities were detected in Jomen-sui of L. edodes sawdust Jomen-culture at 22 days after induction of fruiting. After incubation of 500ng/ml bisphenol A and 4-t-octylphenol with this Jomen-sui, the EDCs were decreased to 17.9% and 23.5%, respectively. Lcc activity in the Jomen-sui was increased until about 3 to 4 weeks after fruiting induction, and then decreased immediately. Jomen-sui could be concentrated about 100- to 200-fold by ultrafiltration method, and then powdered by a freeze-drier without inactivation of Lcc. Lcc activity in the concentrated Jomen-sui was maintained for 100 days at 4℃. Thus, Jomen-sui is applicable for bioremediation as it can remove EDCs, be concentrated, and be preserved for a long time without Lcc inactivation.

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