T0502-2-2 Near-wall nanoparticles perpendicular distribution measured by using evanescent wave

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  • KONO Yuhi
    Graduate School of System Design, Tokyo Metropolitan University
  • KANDA Kensuke
    Exploratory Research for Advanced Tech., Japan Sci. and Tech. Agency
  • OGATA Satoshi
    Graduate School of Sci. and Eng., Tokyo Metropolitan University
  • YANG Ming
    Graduate School of System Design, Tokyo Metropolitan University

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Other Title
  • T0502-2-2 エバネッセント光を用いた壁面近傍におけるナノ粒子の濃度分布の計測(マイクロ・ナノ領域におけるバイオ・熱流体の融合学術創成(2)マイクロ・ナノフルイズ)

Abstract

There has been an increase of research into μTAS. In general, detection with this device is based on a reaction between reactants in the fluid and on the wall of a microchannel. Therefore, it is important to elucidate physical phenomena in the near-wall region of the microchannel to achieve design optimization of the device. The aim of this study is experimental elucidation of single-nanoparticle behavior in microfiuid by observation with an evanescent illumination which allows three-dimensional location measurement of nanoparticles in the near-wall region. Instead of biological molecules, fluorescent nanoparticles (20 nm diameter) were employed for the single particle imaging. Since there are issues of a measurement accuracy due to considerably small diameter of nanoparticles for the imaging and low brightness of a single nanoparticle in the deeper location, an optimal setting of shooting and a novel algorithm of image processing were proposed. The result of experiments showed that perpendicular distribution of nanoparticle concentration exists.

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