S0201-3-5 マイクロパターニング技術を用いた細胞伸展ダイナミクスの評価(マイクロ・ナノバイオメカニクス:細胞生物学への接近(3)界面と接着)

• OKEYO Kennedy Omondi

  京都大・院

• 丸岡 有記子

  京都大・院

• 安達 泰治

  京都大・工

• 北條 正樹

  京都大・工

• 小寺 秀俊

  京都大・工

書誌事項

タイトル別名

• S0201-3-5 Evaluation of Cell Protrusion Dynamics Using Micropatterning Technique

抄録

Actin polymerization-mediated cell protrusion is closely associated with attachment to the substrate and constitutes an important initial step during active cell migration. However, the influence of substrate adhesion property on this fundamental process is less investigated and understood. In order to determine the effect of cell-substrate interaction on cell protrusion dynamics, this study investigated the migration behavior of fish keratocytes on a fabricated glass-substrate consisting of arrays of rectangular fibronectin patterns interleaved with plasma-treated, PLL-g-PEG-coated non-adhesive gaps. We show that both pattern size and interleaving gap length are important limiting factors on lamellipodial protrusion and subsequent migration, and that actomyosin contractility is necessary for stabilizing the lamellipodial actin network for protrusion over the interleaving gaps.

収録刊行物

• 年次大会講演論文集

  年次大会講演論文集 2009.5 (0), 33-34, 2009

  一般社団法人 日本機械学会

キーワード

• Cell migration
• Actin structure
• Protrusion dynamics
• Micropatterning

詳細情報

• CRID: 1390001206063243008
• NII論文ID: 110007866805
• DOI: 10.1299/jsmemecjo.2009.5.0_33
• ISSN: 24331325
• Web Site: https://www.jstage.jst.go.jp/article/jsmemecjo/2009.5/0/2009.5_33/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可