AM05-17-025 ナノ気泡と超音波によるがん細胞への抗がん剤導入に関する研究(生物流体・生体流体5,一般講演)

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  • AM05-17-025 Study on anticancer drug delivery into cancer cells with nanobubbles and ultrasound

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We report on the use of gas-encapsulated nanobubbles (NB) delivered by ultrasound (US) to permeabilize cancer cells and potentiated the cytotoxicity of anti-caner drug (5-FU and cisplatin). We studied 293T (human kidney cells), MCF7 (human breast adenocarcinoma), EMT6 (murine mammary carcinoma) and colon 26 cells (murine rectum carcinoma) using cytotoxicity assays (MTT assay). Plasmid DNA (expressing EGFP or luciferase) was used for optimizing ultrasonic conditions. NB (10% v/v) with US (pressure 0.96MPa) produced significant cytotoxicity not seen with either ultrasound or drug alone. Increasing the duty ratio of US up to 80% further increased cytotoxicity. We proposed a hypothesis that sub-nanobubbles (cavitation bubbles) are produced by collapsed of nanobubbles and shock waves generated by the cavitation bubbles lead to the transient membrane permeability, followed by entry of plasmid DNA and drug. From a theoretical analysis, we obtained the critical shock wave propagation distance from a cavitation bubble that would be necessary for transient membrane permeability.

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