A Compensation Technique of Shaking of Successive Images Taken by the Inclined Centrifuge Microscope

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  • 傾斜遠心顕微鏡で撮影された連続画像のぶれ補正手法(生体の流れ(1),一般講演)

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Abstract

The authors have observed motion of neutrophils on endothelial cells cultured on a flat glass plate using the inclined centrifuge microscope. The successive images of the neutrophils taken by the inclined centrifuge microscope were wobbly due to flatter of rotation of the rotor or vibration of the motor. The shaking of the images led some error in measuring the velocities of individual cells by PTV. The authors have compensated the shaking by sliding the images referring a cross-hair drawn on the glass plate as the index. The cross-hair, however, was blurred because the lines were out of focus of the microscope and the images were dark for the PTV, and thus, they sometimes failed in extracting the lines. Then, in this research, the authors introduced the Hough transformation to extract the lines. Moreover, they detected adherent cells through the images to eliminate remaining shaking of the images. Consequently, the shaking was eliminated within ±1 pixel.

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