P-24 酸素アルカリ処理スギパルプからの同時糖化発酵によるエタノール生産の効率化(ポスターセッション2:技術04,研究発表(ポスター発表)) P-24 Simultaneous saccharification and fermentation process of alkaline treated sugi pulp for effective ethanol production
Japanese cedar (sugi; Cryptomeria japonica) is one of the common woody biomass in Japan. The domestic cumulative dosage of sugi ensures the stable supply of raw material for bioethanol production. Sugi pulp was prepared by soda-anthraquinone cooking followed by oxygen bleaching to increase the susceptibility to cellulase. The simultaneous saccharification and fermentation (SSF) process using general Saccharomyces cerevisiae NBRC 2347 resulted in an ethanol yield of 50% from 10% sugi pulp, 10 FPU/g cellulase preparation at 33℃ after 72 h. On the other hand, the SSF with thermostable yeast BA-16 resulted in an ethanol yield of 78% (36 g/L EtOH) at 40℃ after 72 h of fermentation. The introduction of thermostable yeast to SSF process of sugi pulp resulted in an increase in ethanol yield. Ethanol concentration of 41 g/L was produced using onsite produced enzyme (Trichoderma reesei and Aspergillus tubingensis), substrate loading level of 12.5% at 40℃ after 72 h.
バイオマス科学会議発表論文集 6(0), 100-101, 2011