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First, dissolution behavior of inorganic elements from two mineral natural medicines: "Ryukotsu (Fossilia ossis mastodi)", "Sekkou (Gypsum fibrosum)", and one animal natural medicine: "Borei (Ostreae testa)" was examined using five different solvents: (1) hot water, (2) warm water, (3) warm pH1.2 solution, (4) warm pH4.0 solution and (5) warm p116.8 solution. The dissolution behavior of the calcium in "Ryukotsu", "Borei" and "Sekkou", which mainly consist of calcium salts, differed markedly from each other. Next, the constituents of the two hot water extracts prepared from "Keishi-to" and "Keishi-ka-ryukotsu-borei-to" were compared using inductively coupled plasma-atomic emission spectrometry (ICP-AES) and HPLC analysis. The results show that the "Keishi-ka-ryukotsu-borei-to" extract differed from the "Keishi-to" extract in the constituents from mineral natural medicines as well as plant natural medicines.