4. ジベレリン生合成における13位水酸化反応を阻害する物質(口頭発表,植物化学調節学会第46回大会)

• 酒井 杏奈

  静大院農

• 新間 優子

  静大農

• 大西 利幸

  静大・若手グローバル研究リーダー育成拠点

• 水谷 正治

  神戸大院農

• 平井 伸博

  京大院農

• 轟 泰司

  静大農

書誌事項

タイトル別名

• 4. An inhibitor of the 13-hydroxylases in gibberellin biosynthesis

抄録

The 13-hydroxylation in gibberellin (GA) biosynthesis-the substrate, the enzyme, and the biological significance are still poorly understood. Although recent studies suggest that some CYP714 enzymes catalyze the 13-hydroxylation of ent-kaurenoic acid (KA) or GA_<12>, the biological significance of the 13-hydroxylation remains unclear. The GA receptor, GID1 in Arabidopsis and rice, prefers GA_4 with no hydroxy group at C13 rather than GA_1, the 13-hydroxylated GA. Nevertheless, GA_1 is the major active GA in rice. Why does the 13-hydroxylation exist in GA biosynthesis? Our goal in this study is the development of a selective inhibitor of the 13-hydroxylases in GA biosynthesis as a chemical tool for probing the biological significance of the 13-hydroxylation. We screened inhibitors for Arabidopsis KA 13-hydroxylase, CYP714A, from commercial available azole compounds, and found imazalil (IMZ), which was 30-fold stronger than uniconazole in inhibition of CYP714A. Because IMZ is a fungicide that inhibits fungal CYP51, it is not excluded the possibility that this compound inhibits plant CYP51. Hence, we are constructing the assay system of plant and fungal CYP51 to test the selectivity of IMZ. We are also modifying the structure of IMZ to raise the selectivity.

収録刊行物

• 植物化学調節学会 研究発表記録集

  植物化学調節学会 研究発表記録集 46 (0), 20-, 2011

  一般社団法人 植物化学調節学会

詳細情報

• CRID: 1390282679398819200
• NII論文ID: 110008902413
• DOI: 10.18978/jscrpanb.46.0_20
• ISSN: 21896313
• ISSN: 09191887
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可