96. ヒメツリガネゴケのppGpp合成酵素遺伝子(PpRSH)の機能解析(口頭発表,植物化学調節学会第46回大会)

• 高橋 公咲

  北海道大・院・農

• 佐藤 道大

  北海道大・院・農

• 越智 幸三

  広島工大

• 鍋田 憲助

  北海道大・院・農

書誌事項

タイトル別名

• 96. Functional analysis of RelA/SpoT homologs, PpRSHs, in the moss Physcomitrella patens

抄録

The stringent response is one of the most significant and well-investigated gene regulatory systems in bacteria. The signaling molecule for the stringent response is guanosine 5'-diphosphate 3'-diphosphate (ppGpp). Recently, RelA/SpoT homolog genes (RSHs and CRSHs), which encode ppGpp synthase, have been identified in plants. The accumulation of ppGpp is stimulated by stressful conditions and plant hormones such as abscisic acid or jasmonic acid in plants. A database search revealed the presence of several putative RelA/SpoT homologs in the moss Physcomitrella patens. Two RelA/SpoT homolog genes designated PpRSH1 and PpRSH2, which are involved in the synthesis of ppGpp, were isolated from P patens. Complementary analysis of PpRSH1 and PpRSH2 in E. coli showed that these genes had ppGpp synthetic activity. Additionally recombinant PpRSH1 and PpRSH2 were shown to synthesize ppGpp in vitro. Both proteins were revealed to be localized in chloroplasts. The expression of PpRSH genes were induced upon treatment with abscisic acid or by abiotic stresses such as dehydration and UV irradiation. However jasmonic acid and high concentration of salt did not elevate the expression of PpRSH genes unlike those of RSH genes in flowering plants. The overexpression mutant for PpRSH1 resulted in enhancement of gametophore development. These results suggest that PpRSHs play an important role in stress adaptation and development in P patens.

収録刊行物

• 植物化学調節学会 研究発表記録集

  植物化学調節学会 研究発表記録集 46 (0), 112-, 2011

  一般社団法人 植物化学調節学会

詳細情報

• CRID: 1390001204423162112
• NII論文ID: 110008902505
• DOI: 10.18978/jscrpanb.46.0_112
• ISSN: 21896313
• ISSN: 09191887
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可