マウスES細胞とヒト肝細胞のハイブリッド培養による新しい in vitro 発生毒性試験システムの開発

• 今井 弘一

  大歯大・理工

• 武田 昭二

  大歯大・理工

• 中村 和昭

  国立成育医療センター研・薬剤治療研究部

• 田上 昭人

  国立成育医療センター研・薬剤治療研究部

書誌事項

タイトル別名

• Development of a new in vitro embryotoxicity test system using the hybrid culture of mouse embryonic stem cell and human hepatocyte

抄録

A new in vitro embryotoxicity test is demanded to examine the effects of agents on human metabolic activity. We investigated the hybrid culture of mouse ES cells and three hepatocyte lines according to a 3-D culture protocol with glass fiber. The hepatocyte lines are human and mouse floating cell lines and HepG2. No hepatocyte line was used for a control group. The effects of thalidomide, nonteratogenic in mice, were examined. Thalidomide influenced the metabolic activation of the floating hepatocyte lines as compared with the control group. The protocol will be improved in the future.

収録刊行物

• 日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices

  日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices 31 (2), 139-, 2012-03-25

  一般社団法人日本歯科理工学会

詳細情報

• CRID: 1570854177919101824
• NII論文ID: 110009358534
• NII書誌ID: AA12452694
• ISSN: 18844421
• Web Site: https://search.jamas.or.jp/link/ui/2012206920
• 本文言語コード: ja
• データソース種別:
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