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- 小林 紫苑
- 岩手大学農学部附属寒冷バイオフロンティア研究センター
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- TANINO Karen
- Department of Plant Sciences, University of Saskatchewan
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- 上村 松生
- 岩手大学農学部附属寒冷バイオフロンティア研究センター
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- 河村 幸男
- 岩手大学農学部附属寒冷バイオフロンティア研究センター
書誌事項
- タイトル別名
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- Dynamics of endoplasmic reticulum during freezing in living plant cells
- ショクブツ サイボウ ニ オケル ショウホウタイ トウケツ ドウタイ ノ カンサツ
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Many plants living under subzero temperatures in winter increase freezing tolerance by exposure to non-freezing temperature, which is known as cold acclimation. In cold-acclimated cells, unique cryobehaviors of the plasma membrane and endoplasmic reticulum (ER) have been reported but their physiological meaning or mechanism is largely unknown. Allium fistulosumis a cold-hardy Welshonion which survives winter of-40℃ in Saskatchewan, Canada, andintact cells inthe single epidermal layer, which is easily peeled from leaf sheath, were observed. The cryobehavior of ER in these epidermal cells that were stained with ER-selective fluorescent dye (ER-Tracker) was observed using a confocal fluorescent microscope with cryostage. According to our observations, cold acclimation increased ER volume per cell and extracellular freezing induced ER vesiculation through the breakdown of the ER network. Freeze-induced ER vesicles in cold-acclimated cells were larger and more abundant than those in non-acclimated cells. ER vesiculation may be associated with extracellular calcium because freeze-induced ER vesicles tended to be more abundant in the presence of calcium than in the absence of calcium. Furthermore, ER vesiculation also occurred in Arabidopsis root cells, suggesting a possibility that ER vesiculationis conserved in monocotyledonous and dicotyledonous plants. After thawing, the ER network was recovered only in cold-acclimated cells, suggesting that the dynamics of ER during freeze/thaw cycles are associated with freezing tolerance.
収録刊行物
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- 低温生物工学会誌
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低温生物工学会誌 58 (1), 111-115, 2012
低温生物工学会
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詳細情報
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- CRID
- 1390001205087637120
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- NII論文ID
- 110009457819
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- NII書誌ID
- AN10448734
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- ISSN
- 24241555
- 13407902
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- NDL書誌ID
- 023747057
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可