Simple method of detection of the strains of fluorescent Pseudomonas spp. producing antibiotics, pyrrolnitrin and phloroglucinol

• Tazawa Junko

  Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station

• Watanabe Kenji

  Department of Insect Physiology and Behavior, National Institute of Sericultural and Entomological Science

• Yoshida Hitoshi

  Department of Crop Breeding, Hokkaido National Agricultural Experiment Station

• Sato Mamoru

  Department of Insect Physiology and Behavior, National Institute of Sericultural and Entomological Science

• Homma Yoshihisa

  Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station:(Present office)Department of Farmland Utilization, National Agriculture Research Center

Bibliographic Information

Other Title

• Simple method of detection of the strains of fluorescent Pseudomonas spp.producing antibiotics,pyrrolnitrin and pholoroglucinol

Abstract

Direct colony thin-layer-chromatography (TLC) was applied for the simple and rapid detection of strains producing antifungal substances. In this method, the bacterial colony was directly pasted onto the TLC plate and it was developed twice in the same direction with different solvents. For the detection of strains producing pyrrolnitrin (Pyr), a silica gel TLC plate pasted with the colonies of Pseudomonas fluorescens W8a grown on potato peptone glucose sucrose agar medium was developed firstly with methanol and secondly with a benzene-ethyl acetate mixture. When the plate was dried out and sprayed with Ehrlich's reagent, a clear red spot appeared at Rf=0.74. For 2,4-diacetylphloroglucinol (Phl), the plate pasted with colonies of P. fluorescens LRB3W1 cultured on malt extract agar medium was developed firstly with acetone and secondly with chloroform-methanol. A blue spot was detected on the plate at Rf=0.68 after spraying with Gibbs' reagent. This method was useful to detect the antibiotic-producing strains from wheat roots which were collected from fields continuously cultivated with wheat for a long period of time. This method can also be applied to select antibiotic-deficient strains among the transposon mediated mutants of P. fluorescens W8a.

Journal

• Soil Microorganisms

  Soil Microorganisms 54 (1), 61-67, 2000

  Japanese Society of Soil Microbiology

Keywords

• Pseudomonas fluorescens
• Direct colony TLC
• Pyrrolnitrin
• 2,4-diacetylphloroglucinol

Details

• CRID: 1390282679230071168
• NII Article ID: 110009468015
• NII Book ID: AN00352796
• DOI: 10.18946/jssm.54.1_61
• ISSN: 21896518
• ISSN: 09122184
• NDL BIB ID: 5384577
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5384577
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
• Abstract License Flag: Disallowed