RNAによるアミノ酸の分子非対称性選択の作用機構
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- 田村 浩二
- 東京理科大学・基礎工学部・生物工学科 東京理科大学・総合研究機構 科学技術振興機構・さきがけ
書誌事項
- タイトル別名
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- MECHANISTIC FEATURES OF RNA-DIRECTED MOLECULAR ASYMMETRY OF AMINO ACIDS
- RNA ニ ヨル アミノサン ノ ブンシ ヒタイショウセイ センタク ノ サヨウ キコウ
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<p> Chiral-selective aminoacylation of an RNA minihelix (progenitor of the modern tRNA) could provide a crucial clue to solve the origin of homochirality in a biological system. In this reaction, an amino acid donor (aminoacyl phosphate oligonucleotide) is placed in close proximity to minihelix with the help of a bridging oligonucleotide, which possesses sequences complementary to both donor nucleotide and single-stranded NCCA of minihelix, to accomplish the chiral (L-amino acid)-selective aminoacylation of the minihelix. Here, we propose a molecular mechanism of chiral selectivity based on the mutational analysis of the donor and bridging nucleotides. The selectivity for L-amino acids is dependent on the stereochemistry of RNA. Due to cation coordination and sugar pucker, the side chain of D-amino acids is brought much closer to the terminal adenosine of the minihelix, thereby causing steric hindrance of the D-amino acids during amino acid transfer from the donor nucleotide to minihelix. This mechanism completely explains the result of the original chiral-selective aminoacylation experiment without any contradictions. This selective process may have determined the homochirality of L-amino acids in the putative RNA world.</p><p> </p><p>This article is dedicated to the memory of Dr. Kaoru Harada.</p>
収録刊行物
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- Viva Origino
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Viva Origino 39 (3), 39-44, 2011
生命の起原および進化学会
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詳細情報 詳細情報について
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- CRID
- 1390009073681521408
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- NII論文ID
- 110009470536
- 130008137624
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- NII書誌ID
- AA11148698
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- ISSN
- 13466933
- 09104003
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- NDL書誌ID
- 023889628
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- NDL-Digital
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用可