Augmentation of Arginase 2 Expression in the Human Endometrial Epithelium in the Secretory Phase

  • Tajima Makiko
    Department of Comprehensive Reproductive Medicine, Graduate School, Tokyo Medical and Dental University
  • Harada Tatsuya
    Department of Comprehensive Reproductive Medicine, Graduate School, Tokyo Medical and Dental University
  • Ishikawa Tomonori
    Department of Comprehensive Reproductive Medicine, Graduate School, Tokyo Medical and Dental University
  • Iwahara Yuki
    Department of Comprehensive Reproductive Medicine, Graduate School, Tokyo Medical and Dental University
  • Kubota Toshiro
    Department of Comprehensive Reproductive Medicine, Graduate School, Tokyo Medical and Dental University

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L-Arginine is the common substrate for arginase and nitric oxide synthase (NOS). Arginase converts L-arginine to urea and L-ornithine. L-Ornithine is the principal precursor for the production of polyamines and L-proline, which are required for cell proliferation and collagen synthesis. Endothelial NOS is expressed in the human endometrial glandular epithelium, but the expression and physiological roles of arginase in the human endometrium are not clear. The objective of this study was to investigate the expression and distribution patterns of arginases I (A-I) and II (A-II) in the human endometrium by using immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR), and western blotting. A-I and A-II were detected by immunohistochemistry in human endometrial epithelial cells during the proliferative and secretory phases of the menstrual cycle. RT-PCR showed that A-I and A-II mRNA were expressed in human endometrial tissue. Western blotting analysis results showed the expression of A-II protein. Immunohistochemistry and western blotting results showed that expression levels of A-II were significantly higher in the secretory phase than in the proliferative phase. Increased A-II levels in the secretory phase may be responsible for endometrial growth by increasing polyamines and proline products.

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