Development of macrolide lactone antibiotic brefeldin A fermentation process with Eupenicillium brefeldianum ZJB082702

• WANG Ya-Jun

  Institute of Bioengineering, Zhejiang University of Technology

• XUE Feng

  Institute of Bioengineering, Zhejiang University of Technology

• WU Ye-Fei

  Institute of Bioengineering, Zhejiang University of Technology

• XUE Ya-Ping

  Institute of Bioengineering, Zhejiang University of Technology

• ZHENG Yu-Guo

  Institute of Bioengineering, Zhejiang University of Technology

抄録

In this work, a robust brefeldin A-synthesizing fungus, Eupenicillium brefeldianum ZJB082702, was bred from a Murraya paniculata endophytic fungus E. brefeldianum A1163. Using one-factor-at-a-time experimental design, optimization of media composition for E. brefeldianum ZJB082702 fermenting brefeldin A was conducted. Outcomes indicated that mixed carbon source and mixed nitrogen source were of c ritical importance to brefeldin A fermentation. After 6 d culture in the optimized fermentation media, composed of (gl^<-1>) 13.33 starch, 26.67 glucose, 1.0 yeast extract powder, 1.0 corn steep liquor, 0.5 soybean meal, 0.75 NaNO_3, 2.5 malt extract, 6.0 CaCO_3, 3.0 MgSO_4, 4.0 KH_2PO_4, 1.0×10^<-2> CuSO_4, brefeldin A yield peaked at 1304.7 mgl^<-1>, 648.2 mgl^<-1> in 500 ml baffled flask and 15 l stirred fermentor respectively, formed as a growth associated type of secondary metabolite based on fermentation profile analysis.

収録刊行物

• Journal of bioscience and bioengineering

  Journal of bioscience and bioengineering 114 (3), 262-267, 2012-09-25

  公益社団法人日本生物工学会

キーワード

• Brefeldin A
• Identification
• Eupenicillium brefeldianum
• Breeding
• Fermentation

詳細情報

• CRID: 1572261552806704000
• NII論文ID: 110009517375
• NII書誌ID: AA11307678
• ISSN: 13891723
• 本文言語コード: en
• データソース種別:
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