<Original>A basic study of intraoperative photodynamic therapy for lung cancer : photodynamic therapy for lymphogenous metastases in nude rats

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Background: We designed a new photodynamic therapy (PDT) protocol in which Pheophorbide a (Pba) accumulates in the lymph nodes following local administration around lung cancer tumors, followed by lobectomy and irradiation of the lymph nodes with lasers. As the fi rst step, we evaluated whether administering PDT for metastatic lymph nodes is possible in a rat model.Materials and Methods: Human lung squamous cell carcinoma (RERF-LC-AI) cells were subcutaneously injected into the foot pads of nude rats (F344/rnu-rnu) following X-irradiation. 3 mg/kg of Pba was injected around the tumors in the foot pads, and the Pba concentrations in the popliteal lymph nodes were measured chronologically from the absorption spectrum. The lymph nodes were irradiated with 670-nm light from a diode laser at 180 J/cm2 for 600 seconds and examined histologically.Results: The Pba concentrations in the lymph nodes reached a peak 40 minutes after local application and maintained approximately the same values until six hours. Widespread tumor cell necrosis was histologically evident in the specimens, and the relative proportion of the necrotic areas was 91.3 ± 8.1% (n=6).Conclusions: Our fi ndings suggest that Pba accumulates abundantly in metastatic lymph nodes following local administration into primary tumors and that administering PDT to treat the tumors is possible in a rat model.

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