Ablation of rat substantia nigra may provide a good acute model of Parkinson's disease for stem cell transplantation

• Hirano Shun-ichiro

  Department of Physiology and Graduate School of Dentistry, Osaka Dental University

• Ohashi Momoka

  Department of Orthodontics, Osaka Dental University

• Kakihara Rina

  First Department of Oral and Maxillofacial Surgery, Osaka Dental University

• Suwabe Takeshi

  Department of Physiology and Graduate School of Dentistry, Osaka Dental University

• Fujimoto Tetsuya

  Department of Physiology and Graduate School of Dentistry, Osaka Dental University

• Inoue Hiroshi

  Department of Physiology and Graduate School of Dentistry, Osaka Dental University

• Uchihashi Kenji

  Department of Physiology and Graduate School of Dentistry, Osaka Dental University

• Nishikawa Yasuo

  Department of Physiology and Graduate School of Dentistry, Osaka Dental University

抄録

Using a stereotactic technique, we created a novel, acute model of Parkinson's disease (PD) in 45 female rats by high-frequency electric ablation of bilateral substantia nigra pars compacta with a high-frequency electric coagulator. Targeting was aided by anatomical coordinates from a rat brain atlas. The rats developed persistent symptoms of PD, especially akinesia and postural instability. We tried various surgical protocols. The size and shape of the lesions that were ablated in a typical setting were evaluated. We determined that the optimal surgical parameters for creating a lesion were 1 W of power for 15 sec. The long-term viability of transplanted neural stem/progenitor cells was also examined. We confirmed they were viable for more than 60 days following surgery. As the animal model was designed to suit the study of cell transplantation therapy, it did not utilize neurotoxic chemicals to make lesions, thus avoiding their harmful effects. The technique described here may provide a good acute model of PD for the research of intracerebral cell transplantation. The chronic symptoms of this model and the differentiation control of transplanted cells will be the subject of future study.

収録刊行物

• Journal of Osaka Dental University

  Journal of Osaka Dental University 47 (1), 11-19, 2013

  大阪歯科学会

キーワード

• Parkinson's disease
• Animal model
• High-frequency electric ablation
• Substantia nigra

詳細情報

• CRID: 1390001204960899840
• NII論文ID: 110009603274
• NII書誌ID: AA00704144
• DOI: 10.18905/jodu.47.1_11
• ISSN: 21896488
• ISSN: 04752058
• Web Site: https://search.jamas.or.jp/link/ui/2014246938
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可