両性電解質による細胞凍結保護効果  [in Japanese] Cryoprotective properties of polyampholytes  [in Japanese]

Search this Article

Author(s)

    • 松村 和明 MATSUMURA Kazuaki
    • 北陸先端科学技術大学院大学マテリアルサイエンス研究科、理研、京都大学 School of Meterials Science, Japan Advanced Institute of Science and Technology
    • 林 文晶 HAYASHI Fumiaki
    • 北陸先端科学技術大学院大学マテリアルサイエンス研究科、理研、京都大学 Systems and Structural Biology Center
    • 玄 丞烋 HYON Suong-Hyu
    • 北陸先端科学技術大学院大学マテリアルサイエンス研究科、理研、京都大学 Institute for Medical Science, Kyoto University

Abstract

We developed novel cryoprotective agents from carboxylated ε-poly-L-lysines (COOH-PLLs) for cell cryopreservation as alternatives to dimethyl sulfoxide (DMSO). The polymeric agents showed excellent cell viability profile for most cell types, including human mesenchymal stem cells. We investigated the molecular states and mobility of the solvents and solutes in ice at low temperatures by using solid-state nuclear magnetic resonance (NMR). The NMR data suggested that DMSO and COOH-PLLs differently affect the states and mobility of water and solutes in ice. The former retains the mobility at low temperature, while the latter restricts the mobility by trapping the molecules in soluble aggregates. We propose that cryoprotection of COOH-PLLs is exerted by protecting cells from stresses such as drastic changes in soluble space size and osmotic pressure during freezing.

Journal

  • Cryobiology and cryotechnology

    Cryobiology and cryotechnology 59(1), 23-28, 2013-04-15

    Japanese Society for Cryobiology and Cryotechnology

References:  19

Codes

  • NII Article ID (NAID)
    110009612763
  • NII NACSIS-CAT ID (NCID)
    AN10448734
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    13407902
  • NDL Article ID
    024711813
  • NDL Call No.
    Z17-945
  • Data Source
    CJP  NDL  NII-ELS 
Page Top