Changes in NMR Relaxation Times, Gene Expression and Quality of Seeds : Response to Different Temperature Treatments before and after the Heading Stage of Rice Plants

• MIYAZAKI Masayuki

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University:Fukuoka Agricultural Research Center

• ITO Yumena

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• NONG Thi Hue

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• ISHIBASHI Yushi

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• YUASA Takashi

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University:Department of Agricultural Environmental Sciences, Miyazaki University

• IWAYA-INOUE Mari

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

抄録

We investigated the effects of different temperature treatments on the ^1H-nuclear magnetic resonance (NMR) relaxation times (T_1 and T_2) before and after the heading stage of rice seeds. The T_1 values at temperatures of 25℃/30℃ and 30℃/25℃ before and after the heading stage were lower than those at 25℃/25℃ 21 days after heading (DAH). The T_2 values at 25℃/30℃ and 30℃/25℃ were abruptly shortened at 28 DAH, while that of under 25℃/25℃ treatment was hardly shortened. In addition, dehydration of rice seeds was accelerated by heat stress at 30℃ before or after the heading stage. The changes in T_1 values at 30℃ before or after the heading stage were similar to the changes in the degree of dehydration. These results indicate that T_1 and T_2 are useful diagnostic indicators for the water status during seed ripening of rice plants. A high level of expression of aquaporin gene OsPIP1;1 in seeds at 25℃/30℃ and 30℃/25℃ was detected using DNA microarray analysis at 14 DAH. Seed quality grown under 25℃/30℃ and 30℃/25℃ treatments was inferior to that of seeds under 25℃/25℃ treatment because of the occurrence of white immature kernels. These results suggested that thermal stress either before or after the heading stage led to a reduction in the quality of rice seeds as well as changes in NMR relaxation times and expression of aquaporin genes.

収録刊行物

• 低温生物工学会誌

  低温生物工学会誌 59 (2), 149-155, 2013

  低温生物工学会

キーワード

• Before and after the heading stage
• Gene Expression
• Nuclear magnetic resonance (NMR) relaxation times(T_1,T_2,)
• Rice
• Seed
• Water status

詳細情報

• CRID: 1390001205085836160
• NII論文ID: 110009662033
• NII書誌ID: AN10448734
• DOI: 10.20585/cryobolcryotechnol.59.2_149
• ISSN: 24241555
• ISSN: 13407902
• NDL書誌ID: 024985391
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024985391
• 本文言語コード: en
• データソース種別:
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  • NDL
  • CiNii Articles
  • Crossref
• 抄録ライセンスフラグ: 使用不可