Genome-based authentication of black cohosh ( <i>Cimicifuga racemosa </i>; Ranunculaceae) supplements available in the Japanese markets

DOI Web Site
  • Masada-Atsumi Sayaka
    Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences
  • Onuma Miki
    Graduate School of Pharmaceutical Sciences, Tokyo University of Science
  • Suenaga Emi
    Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences
  • Maruyama Takuro
    Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences
  • Hishida Atsuyuki
    Hokkaido Division, Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation
  • Kiuchi Fumiyuki
    Tsukuba Division, Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation
  • Kobayashi Susumu
    Graduate School of Pharmaceutical Sciences, Tokyo University of Science
  • Goda Yukihiro
    Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences
  • Hakamatsuka Takashi
    Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences

Bibliographic Information

Other Title
  • DNA配列情報を利用したブラックコホシュ国内市場品の基原鑑別
  • Genome-based authentication of black cohosh (Cimicifuga racemosa; Ranunculaceae) supplements available in the Japanese markets

Search this article

Abstract

Black cohosh is one of the most popular herbal medicines which is produced from the roots and rhizomes of Cimicifuga racemosa (Ranunculaceae). Investigations so far have found that some of the black cohosh products were adulterated with other related Cimicifuga species, thus the accurate and convenient technique for the identification of the botanical sources is required. In the present study, we have developed a DNA analytical method to discriminate C. racemosa from six related species by an Amplification Refractory Mutation System (ARMS) analysis. Two kinds of species-specific sense primers were designed on the basis of the nucleotide substitution at position 61 on the trnL gene among the seven species, and the presence of 284 bp fragments was detected upon PCR amplification. The resultant fragments were species-specific when this method was applied for the referential plant samples. Commercial black cohosh products were then tested in the same way and the result indicated that three of the eight products were not derived from C. racemosa. Moreover, TLC and HPLC analyses were performed for marker compounds in sixteen commercial products to determine the reliability of the ARMS analysis. These metabolic analyses completely followed the results of the ARMS analysis and strongly suggested its usefulness.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top