Characterization of Glycosylphosphatidylinositol-Anchored Ceruloplasmin Enriched in the Apical Plasma Membrane of Rat Hepatocytes

機関リポジトリ HANDLE Web Site

この論文をさがす

抄録

Aim : Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because of its ferroxidase activity. It is expressed as soluble (sCp) or glycosylphosphatidylinositol-anchored ceruloplasmin (GPI-Cp) form ; the former is primarily synthesized in the liver, and the latter is primarily found in the brain. Although recent studies reported GPI-Cp expression on hepatocytes, little is known regarding its presence in specific liver cell compartments and its possible involvement in liver pathophysiology. This study aimed to characterize the distribution of GPI-Cp in liver cells and specifically in the apical part of the plasma membrane. Methods : We assessed GPI-Cp expression in the liver using immunohistochemistry and immunoblotting techniques. Furthermore, we isolated apical and basolateral membrane fraction from the total liver membrane using sucrose discontinuous gradient centrifugation, and GPI-Cp were detected using immunoblotting. Results : GPI-Cp was detected in purified apical membranes of rat liver cells. Immunoreactive Cp protein was released after incubation with phosphatidylinositol-specific phospholipase C, and the free protein demonstrated ferroxidase activity. Conclusion : These findings suggest that majority of GPI-Cp present in the liver is primarily located on the apical surface of cells because of transcytosis.

収録刊行物

  • 秋田医学

    秋田医学 40 (3/4), 163-173, 2014-03-31

    秋田医学

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ