O-405 バイオマス由来水素または合成ガスからの有用物質生産を目的とした好熱性微生物の遺伝子組換技術の開発(ガス・ケミカル,Oral Session4)

DOI

書誌事項

タイトル別名
  • O-405 Development of genetic transformation system in thermophilic acetogen to produce useful materials from H_2-CO_2 or syngas from biomass

抄録

To develop a genetic transformation system for M. thermoacetica ATCC39073, the uracil auxotrophic strain dpyrF was constructed by disrupting pyrF for orotate monophosphate (OMP) decarboxylase. To test heterologous gene expression in dpyrF, the lactate dehydrogenase (LDH) gene (T-ldh) from Thermoanaerobacter pseudethanolicus ATCC33223 was electroporated into dpyrF with a promoter of the glyceraldehyde-3-phosphate dehydrogenase (G3PD) gene of M. thermoacetica ATCC39073. The resulting transformant successfully produced 6.8 mM of lactate from fructose. To develop a transformation system without auxotrophy, a thermostable kanamycin resistant gene (kan^r) derived from the plasmid that Streptococcus faecalis harbored was introduced into M. thermoacetica ATCC39073. By using kan^r with promoter of endogenous G3PD gene as a selection marker, we succeeded at the transformation. Using the developed system, we successfully constructed acetate-deficient, lactate producing mutant by deleting phosphotransacylase gene candidates and introducing T-ldh.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390001205786750848
  • NII論文ID
    110009820156
  • DOI
    10.20550/jiebiomassronbun.9.0_40
  • ISSN
    24238341
    24238333
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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