マウス骨芽細胞MC3T3-E1の石灰化能に与えるPQQの影響

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  • 比嘉 由美子
    琉球大学農学部亜熱帯生物資源科学科発酵・生命科学分野
  • 橘 信二郎
    琉球大学農学部亜熱帯生物資源科学科発酵・生命科学分野

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タイトル別名
  • Effect of pyrroloquinoline quinone on mineralization in murine osteoblastic MC3T3-E1 cells
  • マウス コツガ サイボウ MC3T3-E1 ノ セッカイカノウ ニ アタエル PQQ ノ エイキョウ

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抄録

Pyrroloquinoline quinone (PQQ), a redox cofactor in several bacteria, has recently attracted attention as a vitamin-like biofactor with various bioactivities reported in mammals. However, there is little information regarding the effects of PQQ on bone metabolism. Thus, we examined the effects of PQQ on mineralization using murine osteoblastic MC3T3-E1 cells to predict its possible actions on bone formation in vivo. In undifferentiated cells, PQQ (10^<-4> M) markedly decreased cell viability and proliferation rate, but these cytotoxic effects were moderately altered in differentiated cells. In differentiated cells cultured for 14 days, PQQ reduced mineralization in a concentration-dependent manner. Alkaline phosphatase (ALP) activity was enhanced in differentiated osteoblastic cells treated with low concentrations of PQQ (10^<-10> and 10^<-6> M), but the expression level of ALP mRNA in the PQQ-treated osteoblastic cells was similar to or lower than that in the control. The mRNA expression of osteocalcin, bone matrix noncollagenous protein, tended to decrease with an increase of PQQ concentrations, even though the COL1A1 mRNA expression levels showed no significant differences between control and PQQ-treated cells. Our findings demonstrate a negative role of PQQ on mineralization during osteoblast differentiation in vitro which is independent of ALP activity.

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  • ビタミン

    ビタミン 88 (8), 396-404, 2014

    公益社団法人 日本ビタミン学会

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