PDMS上に接着させた細胞の凍結保存におけるDimethyl sulfoxide濃度の検討

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タイトル別名
  • Estimation of Dimethyl Sulfoxide Concentration for Cryopreservation of Cell Attached on PDMS
  • PDMS ジョウ ニ セッチャク サセタ サイボウ ノ トウケツ ホゾン ニ オケル Dimethyl sulfoxide ノウド ノ ケントウ

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Cells have a high molecular discerning ability. Recently, taking advantage of this ability as a kind of biosensors, cells attached on matrix have been developed in the field of biosensors. In the previous study, we reported cells attached to the glass matrix can be cryopreservated using 20v/v% concentration of DMSO as a cryoprotective agent, which usually allows 10v/v% DMSO concentration in cryovials. However, Poly-Dimethyl Siloxane (PDMS) is preferably used as a matrix for biosensors instead of the glass matrix. In the present study, we investigated cryopreservation of HepG2 cells attached on PDMS matrix by applying DMSO as a cryoprotectctive agent. As a result, the rate of cell adhesion increased with the DMSO concentration. Cell viability was highest at 20v/v% DMSO concentration. However, 30v/v% DMSO concentration had a cytotoxic effect to cell viability. Thus, similar to the glass matrix, 20v/v% DMSO concentration is suitable for the cryopreservation matrix.

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