2光子励起顕微鏡を用いた生体深部イメージングと光顕微操作(<特集>ライブセルイメージング)

• 水多 陽子

  名古屋大学大学院理学研究科:JST ERATO東山ライブホロニクスプロジェクト

• 栗原 大輔

  名古屋大学大学院理学研究科:JST ERATO東山ライブホロニクスプロジェクト

書誌事項

タイトル別名

• In vivo deep imaging and optical manipulation by two-photon excitation microscopy(<Feature Articles>Live-cell imaging)
• 2光子励起顕微鏡を用いた生体深部イメージングと光顕微操作
• 2 ミツコレイキケンビキョウ オ モチイタ セイタイ シンブ イメージング ト ヒカリケンビソウサ

抄録

Cell-to-cell communication plays an important role in development, reproduction, and environmental responses by coordinating the cell activity in multicellular organisms. In plant development, cell-to-cell communication is especially crucial because organogenesis is a postembryonic and continuous process. However, it is difficult to reveal the dynamics of intercellular communications due to the limitation of accessibility within several cell layers in plants. Recently, two-photon excitation microscopy with near-infrared femtosecond pulse laser provides a non-invasive tool for deep imaging. In this review, we discuss recent deep imaging containing the preparation of specimens and microscopic techniques. Moreover, optical manipulation technique with near-infrared ultrafast pulse laser enables us to disrupt the cell and the protein at the single-cell level. Combination of in vivo deep imaging and optical manipulation, which allow spatial and temporal control of cell activity, provides a new view of the cell-to-cell communication in plant field.

収録刊行物

• 植物の生長調節

  植物の生長調節 49 (2), 96-103, 2014

  一般社団法人 植物化学調節学会

詳細情報

• CRID: 1390001205086555648
• NII論文ID: 110009913068
• NII書誌ID: AA11550064
• DOI: 10.18978/jscrp.49.2_96
• ISSN: 21896305
• ISSN: 13465406
• NDL書誌ID: 025986240
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025986240
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可