OS0712 マルチチャネルデバイスを用いた細胞遊走観察と牽引力計測

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  • OS0712 Cell migration observation and traction force measurement using multichannel device

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Cell migration plays an important role in many physiological and pathological events, however, the mechanism of collective cell migration has not been well understood. This study performs traction force microscopy on migrating NIH 3T3 fibroblasts using a multichanel device. The device consists of a reservoir for cell culture and 24 microchannels with arrays of micropillars for traction force microscopy. Here, the effect of anisotropic properties in stiffness of substrate on collective cell migration is tested with ellipsoidal cross section of micropillars. The results showed that cells migrated into the microchannels and produced higher traction forces in the direction of major axis of micropillars. This finding suggests that cell can sense the stiffness of substrate and produce traction forces depending on the stiffness, resulting in difference in cell migration rate.

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