20724 静水圧負荷に応答する軟骨細胞のシグナル伝達

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  • 20724 Signaling of Chondrocytes in Response to Hydrostatic Pressure Loading

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Mechanical stimulation has attracted attention as a stimulus for optimal cultivation of regenerated cartilage, but the signal transduction mechanism has not yet been clarified. Though there are reports that differentiation genes, like Sox9, are up-regulated by loading hydrostatic pressure (HP) to chondrocytes, it is unclear which upstream signals respond to HP. We used ATDC5, chondroprogenitor cells derived from mice, to find HP-sensitive molecules and signaling pathways, and quantify the activation of signaling proteins by western blotting. Under 25 MPa, CREB and ERK1/2 activation were observed after 5 minutes. Loading HP with the addition of galleon, a molecule that inhibits signaling triggered by the βγ subunits of heterotrimeric G proteins, ERK1/2 activation was still observed, showing that ERK activation is independent of those subunits. For further studies, we will use inhibitors for several possible mechanosensitive pathways to explore upstream signal transduction.

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