J028023 細胞骨格張力が単離腱細胞の代謝能へ与える影響

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  • J028023 Modulation of Cytoskeletal Tension Influences Cell Metabolism of Isolated Tenocytes

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Tendon is subjected to dynamic mechanical loading in vivo and thus effects of cyclic tensile loading on tenocyte metabolism have been well characterized. However, the relationship between cell metabolism and cytoskeletal tension has not been studied in detail. Elastic micropillars made from silicone elastomer (PDMS) have been proposed to measure cellular traction forces which reflect cytoskeletal tension. Micropillar substrates with three different stiffness were prepared by changing the height of the pillars. First, to examine the effects of the substrate stiffness, tenocytes isolated from bovine tendons were seeded on the micropillar substrates. At the end of 24 hrs incubation period, cellular traction forces were determined. Real-time qPCR was also performed to examine the expressions of type I collagen (anabolic gene) and MMP-1 (catabolic gene) mRNA from tenocytes. In addition, to examine the role of acto-myosin contractility, tenocytes were treated with myosin II inhibitor, blebbistatin at 20 hrs. It was found that there were significant increases in cellular traction forces with increasing stiffness of micropillar substrates. Although there were no significant differences in the expression level of type I collagen among the three substrates, significant increases were observed in the expression level of MMP-1 with decreasing in stiffness of substrate. Moreover, traction forces decreased and the expression level of MMP-1 increased with the treatment of blebbistatin. This may indicate that intracellular cytoskeletal tension, driven by acto-myosin contractility, could strongly influence tenocyte catabolism rather than anabolism.

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  • 年次大会

    年次大会 2012 (0), _J028023-1-_J028023-4, 2012

    一般社団法人 日本機械学会

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