Accurate enumeration and identification of Testacea (Protozoa, Rhizopoda) in forest soil using scanning electron microscopy

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A new procedure is used for separating Testacea from soil, based on dispersion, sequential centrifugation-settling and subsequent filtration of the soil suspension. Combined with scanning electron microscopy (SEM), this procedure allows accurate enumeration of Testacea, with a recovery rate of 90%) and more precise counting (RSD: 5%) of Testacea than in previous works.

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