Bacteriophage control of Pseudomonas plecoglossicida infection in ayu, Plectoglossis altivelis
書誌事項
- タイトル別名
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- Bacteriophage control of Pseudomonas plecoglossicida infection in ayu.
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Two previously isolated phages were used to examine the therapeutic effects against Pseudomonas plecoglossicida infection in ayu Plecoglossus altivelis. Phage PPp-W4 (Podoviridae) inhibited the in vitro growth of P. plecoglossicida more effectively than Phage PPpW-3 (Myoviridae), and a mixture (PPpW-3/W-4) of the 2 phages exhibited the highest inhibitory activity. In phage therapy experiments, ayu were fed P. plecoglossicida-impregnated feed (107 CFU fish-1) and then fed phage-impregnated feed (107 PFU fish-1). Mortalities of fish receiving PPpW-3, PPpW-4, PPpW-3/W-4, and a control fish receiving no phages were 53.3, 40.0, 20.0 and 93.3%, respectively. Phage (PPpW-3/W-4)-receiving fish also showed high protection against water-borne infection with P. plecoglossicida. In a field trial, when phage (PPpW-3/W-4)-impregnated feed was administered to ayu in a pond where the disease occurred naturally, daily mortality of fish decreased at a constant level (5% d-1) to one-third after a 2 wk period. The causal relationship of phages in this phenomenon was verified by the long-lasting appearance of administered phages in the kidneys of the fish, and a disappearance of P. plecoglossicida from apparently healthy fish. Neither phage-resistant organisms nor phage-neutralizing antibodies were detected in diseased fish or apparently healthy fish, respectively. These results indicate the potential for phage control of the disease.
収録刊行物
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- Diseases of Aquatic Organisms
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Diseases of Aquatic Organisms 53 (1), 33-39, 2003-01-22
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詳細情報 詳細情報について
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- CRID
- 1050577740963433728
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- NII論文ID
- 120001048998
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- NII書誌ID
- AA10443976
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- ISSN
- 16161580
- 01775103
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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