Bone morphogenetic protein-2 down-regulates miR-206 expression by blocking its maturation process

• Sato, Mari M.

• Nashimoto, Masayuki

• Katagiri, Takenobu

• Yawaka, Yasutaka

• Tamura, Masato

抄録

MicroRNAs (miRNAs) are small non-coding RNAs that are emerging as important post-transcriptional gene regulators. miR-206 is unique in that it is expressed only in skeletal muscle, including the myoblastic C2C12 cell line. In C2C12 cells, miR-206 expression was reduced dramatically after bone morphogenetic protein (BMP)-2 treatment. The down-regulation of miR-206 expression was also observed after co-transfection with constitutively-active Smad1 and Smad4, which are the intracellular signaling molecules of the BMP pathway. BMP-2 also reduced miR-206 expression in the presence of α-amanitin in a similar manner to that in the absence of α-amanitin. Moreover, the expression of pri-miR-206 was increased upon BMP-2 treatment for 6 hours compared to that in the absence of BMP-2. These results suggested that BMP-2 down-regulates miR-206 expression at the post-transcriptional level, by inhibiting the processing of pri-miR-206 into mature miR-206, and that BMP-2 could regulate miRNA biogenesis by a novel mechanism.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 383 (1), 125-129, 2009-05-22

  Academic Press

キーワード

• miR-206
• miRNA processing
• bone morphogenetic protein
• Smad
• myoblasts

詳細情報

• CRID: 1050282813977590144
• NII論文ID: 120001243503
• NII書誌ID: AA00564395
• ISSN: 10902104
• ISSN: 0006291X
• HANDLE: 2115/38314
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles