Activation of the Salicylic Acid Signaling Pathway Enhances Clover yellow vein virus Virulence in Susceptible Pea Cultivars

• Atsumi, Go

• Kagaya, Uiko

• Kitazawa, Hiroaki

• Nakahara, Kenji Suto

• Uyeda, Ichiro

Bibliographic Information

Other Title

• Activation of the Salicylic Acid Signaling Pathway Enhances <i>Clover yellow vein virus</i> Virulence in Susceptible Pea Cultivars

Abstract

The wild-type strain (Cl-WT) of Clover yellow vein virus (ClYVV) systemically induces cell death in pea cv. Plant introduction (PI) 118501 but not in PI 226564. A single incompletely dominant gene, Cyn1, controls systemic cell death in PI 118501. Here, we show that activation of the salicylic acid (SA) signaling pathway enhances ClYVV virulence in susceptible pea cultivars. The kinetics of virus accumulation was not significantly different between PI 118501 (Cyn1) and PI 226564 (cyn1); however, the SAresponsive chitinase gene (SA-CHI) and the hypersensitive response (HR)-related gene homologous to tobacco HSR203J were induced only in PI 118501 (Cyn1). Two mutant viruses with mutations in P1/HCPro, which is an RNA-silencing suppressor, reduced the ability to induce cell death and SA-CHI expression. The application of SA and of its analog benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH) partially complemented the reduced virulence of mutant viruses. These results suggest that high activation of the SA signaling pathway is required for ClYVV virulence. Interestingly, BTH could enhance Cl-WT symptoms in PI 226564 (cyn1). However, it could not enhance symptoms induced by White clover mosaic virus and Bean yellow mosaic virus. Our report suggests that the SA signaling pathway has opposing functions in compatible interactions, depending on the virus-host combination.

Journal

• Molecular Plant-Microbe Interactions

  Molecular Plant-Microbe Interactions 22 (2), 166-175, 2009-02

  APS Press in cooperation with the International Society for Molecular Plant-Microbe Interactions

Keywords

• resistance gene

Details

• CRID: 1050845763931077376
• NII Article ID: 120001364373
• DOI: 10.1094/mpmi-22-2-0166
• ISSN: 19437706
• ISSN: 08940282
• HANDLE: 2115/38634
• Web Site: https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-22-2-0166
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN