書誌事項
- タイトル別名
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- Metabolism of 2,2',5,5'-Tetrachlorobiphenyl (CB52) by Rabbit Liver Microsomes
- 2 2 5 5 4 エンソカ ビフェニル CB52 ノ ウサギ カン ミクロゾーム ニ ヨル タイシャ
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Our preceding studies have reported that 2,2',5,5'-tetrachlorobiphenyl (tetraCB)(CB52) is mainly metabolized to 3-hydroxy (OH)-metabolite by phenobarbital (PB)-inducible cytochrome P450 (P450) isoforms such as CYP2B1 and CYP2B18. In this study, the metabolism of CB52 by liver microsomes of untreated and PB-treated rabbits was investigated. Rabbit liver microsomes produced mainly 3-OH- and 4-OH-metabolites (M-1 and M-2) at an equal extent and two other metabolites (M-3 and M-4) and also that phenobarbital (PB) treatment accelerated the formation of all these metabolites. M-3 was assumed to OH-tetraCB by GC-MS. Another metabolite, M-4, was determined to 3,4-diOH-CB52 by GC-MS and 1H-NMR. Addition of antiserum against CYP2B4, a constitutive and PB-inducible rabbit P450 isoform, to a microsomal incubation system resulted in almost complete inhibition of the formation of 3-OH-, 4-OH- and 3, 4-diOH-metabolites. These results suggest that CYP2B4 plays an important role in CB52 metabolism in rabbit liver.
収録刊行物
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- 福岡醫學雜誌
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福岡醫學雜誌 100 (5), 200-209, 2009-05-25
福岡医学会
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詳細情報 詳細情報について
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- CRID
- 1390290699740018048
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- NII論文ID
- 120001390933
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- NII書誌ID
- AN00215478
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- DOI
- 10.15017/14915
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- HANDLE
- 2324/14915
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- NDL書誌ID
- 10409195
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- ISSN
- 0016254X
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- PubMed
- 19588850
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用可