Basic fibroblast growth factor promotes the generation of microtubule-associated protein 2-positive cells from microglia

• 新留, 徹広

• Nonaka, Hideki

• Akaike, Akinori

• Kihara, Takeshi

• Sugimoto, Hachiro

抄録

We recently demonstrated that microglia as multipotential stem cells give rise to microtubule-associated protein 2 (MAP2)-positive and glial fibrillary acidic protein (GFAP)-positive cells and that microglia-derived MAP2-positive cells possess properties of functional neurons. In this study, we investigated the role of fibroblast growth factor (FGF) signaling in the molecular mechanism underlying the generation of microglia-derived MAP2-positive and GFAP-positive cells. Real-time quantitative PCR analyses demonstrated that mRNA levels of a family of three FGF receptors, Fgfr1-3, were upregulated in microglia treated with 70% fetal bovine serum (FBS). Immunocytochemical analyses demonstrated that basic FGF (bFGF) promoted the generation of microglia-derived MAP2-positive and GFAP-positive cells, and the FGF receptor tyrosine kinase inhibitor SU5402 and the MEK inhibitor PD98059 both inhibited this process. Western blot analyses demonstrated that bFGF increased phosphorylated ERK1/2 levels without altering total ERK1/2 levels. These results suggest that bFGF promotes the generation of microglia-derived MAP2-positive and GFAP-positive cells via FGF receptors and the ERK-MAP kinase pathway.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 390 (3), 1018-1022, 2009-12-18

  Elsevier BV

キーワード

• Basic fibroblast growth factor
• Differentiation
• Fibroblast growth factor receptor
• Glial fibrillary acidic protein
• Microglia
• Microtubule-associated protein 2

詳細情報

• CRID: 1050845760539227648
• NII論文ID: 120001749557
• NII書誌ID: AA00564395
• ISSN: 0006291X
• HANDLE: 2433/88960
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles