Construction of Several Delection Mutants for Genes Involved in Biofilm Formation and Recovery of Heat-injured Salmonella: Delta {agfA} and Delta {bcsA} Mutants of Salmonella Enteritidis; Delta {ahpC}, Delta {ahpF}, and Delta {katG} Mutants of S. Typhimurium; and Delta {rpoE}, Delta {rpoH}, and Delta {rpoS} Mutants of S. Enteritidis and S. Typhimurium

• 本城 賢一

  九州大学大学院農学研究院生物機能科学部門食品バイオ工学講座食品衛生化学分野

• Hashimoto Yoshikazu

  Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Shimotsu Satoshi

  Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Wen Hsu-Ming

  Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Kiriki Madoka

  Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Naito Kimitaka

  Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Tokugawa Mio

  Program of Food Science and Technology, Course of Applied Biological Science, Department of Bioresource and Environmental Sciences, Kyushu University

• Satake Emiko

  Program of Food Science and Technology, Course of Applied Biological Science, Department of Bioresource and Environmental Sciences, Kyushu University

• 小林 弘司

  九州大学大学院農学研究院生物機能科学部門食品バイオ工学講座食品衛生化学分野

• 宮本 敬久

  九州大学大学院農学研究院生物機能科学部門食品バイオ工学講座食品衛生化学分野

抄録

Salmonella is one of major food-poisoning bacteria. In food companies, controlling the bacterial growth is critically important to continue provision of good products to customers. At first, to study the attachment of the bacteria to food materials and final products, we focused on biofilm formation that is thought to be one of the reasons of the attachment. Thus, the genes related in formation of biofilm were also focused on, and Delta {agfA} and Delta {bcsA} mutants of Salmonella Enteritidis were constructed by using 2-step PCR and pKOBEGA helper plasmid. Secondary, we focused on heat-injured Salmonella after sublethal heat treatment as previously reported (Kobayashi et al., 2005). The heat-injured Salmonella showed expression of several specific genes to recover the bacterial functional state as well as other bacteria. To clarify the mechanism of the recovery, several gene mutants (Delta {ahpC}, Delta {ahpF}, and Delta {katG} mutants of Salmonella Typhimurium, and of Delta {rpoE}, Delta {rpoH}, and Delta {rpoS} mutants of S. Enteritidis and S. Typhimurium) were constructed.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 54 (2), 421-431, 2009-10-29

  九州大学大学院農学研究院

キーワード

• Agronomy and Crop Science
• Biotechnology

詳細情報

• CRID: 1390572174638183424
• NII論文ID: 120001766569
• NII書誌ID: AA00247166
• DOI: 10.5109/16125
• HANDLE: 2324/16125
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles