Antibiotics for Agrobacterium Elimination in Apple Transformation

DOI IR JASI Web Site Web Site 4 Citations 24 References

Bibliographic Information

Other Title
  • リンゴの形質転換におけるＡｇｒｏｂａｃｔｅｒｉｕｍ除菌用抗生物質の検討
  • リンゴ ノ ケイシツ テンカン ニ オケル Agrobacteriumジョキンヨウ コウセイブッシツ ノ ケントウ

Search this article

Abstract

We investigated several antibiotics including carbenicillin (CBPC), clavulanic acid/amoxicillin (CVA/AMPC), cefotaxime (CTX), meropenem (MEPM), vancomycin (VCM) and doxycycline (DOXY) for Agrobacterium elimination during apple transformation. The growth of Agrobacterium tumefaciens EHA101 was suppressed at 1,500 mg·L−1 of CBPC, 750 mg<orgname lang = "ja">·</orgname>L−1 of CVA/AMPC, less than 500 mg·L−1 of CTX, 50 mg·L−1 of MEPM and 200 mg<orgname lang = "ja">·</orgname>L−1 of DOXY, but growth was not suppressed at 1,500 mg·L−1 of VCM. In the shoot length, there was no difference among antibiotic-free, CBPC, CTX and VCM treatments. However, the shoot length in the high-concentration treatment of CVA/AMPC and DOXY was significantly shortened. However, the number of shoots increased as the concentration of MEPM rose. In CTX treatment, the shoot regeneration rate from leaf segments was lowered in comparison with antibiotic-free treatment; however, the regeneration rate was maintained to some degree even after high-concentration CTX treatment. The regeneration rate from leaf segments after MEPM treatment was superior to the antibiotic-free treatment. After DOXY treatment, leaf segments did not grow at all and finally died. These findings indicated that bacterial cell wall synthesis inhibitors such as CTX and MEPM were effective in eliminating Agrobacterium.<br>

Journal

Citations (4)*help

See more

References(24)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top