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- 斉藤, 泰
- 長崎大学医学部泌尿器科教室
書誌事項
- タイトル別名
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- Studies on prostatic phosphatase. (1) Purification and immunologic certification of human prostatic phosphomonoesterase
- ゼンリツセン ノ カイリン コウソ ニ カンスル ケンキュウ 1 ヒト ゼンリツセン フォスフォモノエステラーゼ ノ セイセイ ト ソノ メンエキガクテキ ショケン
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Prostatic phosphomonoesterase (prostatic PMEase) was purified by London's and Ostrowski's methods as follows. The first step is fullers' earth adsorption, the second step is fractionation with ammonium sulfate, the third step is DEAE cellulose column chromatography and the fourth step is rechromatography of a fraction obtained by the third step. The purified prostatic PMEase was in homogeneous form electrophoretically and ultracentrifugally. Four distinct peaks of protein were obtained by the DEAE cellulose column chromatography and the PMEase activity was exhibited in the three peaks except the first peak. Each peak of the purified materials by the DEAE cellulose column chromatography was examined by immunoelectrophoresis with antihuman prostatic PMEase serum of the rabbit. The PMEase of peak II migrates to the cathode, that of peak III migrates slightly to the anode and that of peak IV migrates to the anode more than that of peak III. It was found by immunoelectrophoresis that the prostatic PMEase which was in homogeneous form electrophoretically and ultracentrifugally, contained always siderophilin. Each PMEase fraction of the three peaks was examined on t h e enzymatic properties such as optimal pH., Michael's constant and inhibition test. The enzymatic properties of each PMEase fraction were slightly different each other.
収録刊行物
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- 泌尿器科紀要
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泌尿器科紀要 12 (8), 760-778, 1966-08
京都大学医学部泌尿器科学教室
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詳細情報 詳細情報について
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- CRID
- 1050001201693801472
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- NII論文ID
- 120002141006
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- NII書誌ID
- AN00208315
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- ISSN
- 00181994
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- HANDLE
- 2433/113007
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- NDL書誌ID
- 8425321
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- 本文言語コード
- ja
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- 資料種別
- departmental bulletin paper
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- データソース種別
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- IRDB
- NDL
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