A novel copper(II) coordination at His186 in full-length murine prion protein

• Watanabe, Yasuko

• Hiraoka, Wakako

• Igarashi, Manabu

• Ito, Kimihito

• Shimoyama, Yuhei

• Horiuchi, Motohiro

• Yamamori, Tohru

• Yasui, Hironobu

• Kuwabara, Mikinori

• Inagaki, Fuyuhiko

• Inanami, Osamu

抄録

To explore Cu(II) ion coordination by His186 in the C-terminal domain of full-length prion protein (moPrP), we utilized the magnetic dipolar interaction between a paramagnetic metal, Cu(II) ion, and a spin probe introduced in the neighborhood of the postulated binding site by the spin labeling technique (SDSL technique). Six moPrP mutants, moPrP(D143C), moPrP(Y148C), moPrP(E151C), moPrP(Y156C), moPrP(T189C), and moPrP(Y156C,H186A), were reacted with a methane thiosulfonate spin probe and a nitroxide residue (R1) was created in the binding site of each one. Line broadening of the ESR spectra was induced in the presence of Cu(II) ions in moPrP(Y148R1), moPrP(Y151R1), moPrP(Y156R1), and moPrP( T189R1) but not moPrP(D143R1). This line broadening indicated the presence of electron–electron dipolar interaction between Cu(II) and the nitroxide spin probe, suggesting that each interspin distance was within 20 Å. The interspin distance ranges between Cu(II) and the spin probes of moPrP(Y148R1), moPrP(Y151R1), moPrP(Y156R1), and moPrP(T189R1) were estimated to be 12.1 Å, 18.1 Å, 10.7 Å, and 8.4 Å, respectively. In moPrP(Y156R1,H186A), line broadening between Cu(II) and the spin probe was not observed. These results suggest that a novel Cu(II) binding site is involved in His186 in the Helix2 region of the C-terminal domain of moPrPC.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 394 (3), 522-528, 2010-04-09

  Elsevier

キーワード

• Prion protein
• Cu(II)
• C-terminal domain
• ESR
• SDSL
• MTSSL
• Interspin distance
• Dipolar interaction
• Histidine residue

詳細情報

• CRID: 1050282677654429696
• NII論文ID: 120002148672
• NII書誌ID: AA00564395
• ISSN: 10902104
• ISSN: 0006291X
• HANDLE: 2115/43092
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles