Estimation of Self-incompatibility S Genotypes of Citrus Cultivars and Plants Based on Controlled Pollination with Restricted Number of Pollen Grains

• Ngo Binh Xuan

  Departent of Agricultural Biotechnology, Faculty of Agronomy, Thai Nguyen University of Agriculture and Forestry

• 若菜 章

  九州大学大学院農学研究院植物資源科学専攻農業植物学講座園芸学分野

• Kim Jung Hee

  Laboratory of Horticultural Science, Division of Agricultural Botany, Department of Plant Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• 森 智代

  九州大学大学院生物資源環境科学府植物資源学専攻農業植物科学講座園芸学分野

• 酒井 かおり

  九州大学大学院農学研究院植物資源科学専攻農業生産生態学講座農業生産生態学分野

抄録

Self-incompatibility S genotypes of 29 Citrus cultivars were estimated by the observation of pollen tube behavior in the lower one-third of styles with the aid of an epifluorescent microscope 7 or 8 days after controlled pollination with restricted number of pollen grains (about 100) on their stigmas. In eight crosses considered to be fully compatible on the basis of segregation distortion of glutamate oxaloacetate transaminase (GOT) isozymes in the progenies of cultivars used for the crosses, the rate of pollen tubes reaching the style base ranged from 7.7% to 24.6% of pollen grains putting on the stigmas, while in twelve crosses considered to bo semi-compatible the rate of pollen tubes reaching the style base ranged from 1.6% to 19.3% of pollen grains on the stigmas. The rates were slightly different in different pistillate parents. Twenty cultivars whose genotypes were not determined were pollinated with restricted number of‘Banpeiyu’(S_1 S_2) pollen grains and their genotypes were predicted by the pollen tube behaviors. Similarly, 13 controlled crosses were carried out with restricted number of pollen grains and their S genotypes were determined based on the number of pollen tubes reaching the style base. From these results, it became clear that the predicted S genotypes of the cultivars are well correspond with pollen tube behaviors in full or semi compatible crosses and, eventually, it was estimated that 15 cultivars have either S_1 or S_2 allele that‘Banpeiyu’pummelo has.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 55 (1), 67-72, 2010-02-26

  九州大学大学院農学研究院

キーワード

• Agronomy and Crop Science
• Biotechnology

詳細情報

• CRID: 1390009224684738816
• NII論文ID: 120002223676
• NII書誌ID: AA00247166
• DOI: 10.5109/17802
• HANDLE: 2324/17802
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles