Tissue typing by unidirectional mixed lymphocyte culture. I. Demonstration of the cumulative effect of non-H-2 antigens in mice by cultured lymphocytes mixed with cell homogenate

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<p>1. The mixed lymphocyte culture test where live lymphocytes of female mice are cultured with supersonicated cell homogenate of isogeneic male mice does not reflect the difference in H-Y histocompatibility antigen alone. 2. When non-H-2 antigens are cumulated in various combinations, the rate of blastformation becomes greater than the combination of C3H ♀ themselves. 3. When non-H-2 antigens and H-Y antigens are added in various combinations, the rate of blastformation becomes significantly higher than in the combination of C3H ♀ themselves.</p>

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