An electronmicroscope study of liver cell in carbon tetrachloride intoxication,significance of "opaque"

DOI 機関リポジトリ Web Site Web Site

書誌事項

タイトル別名
  • An electron microscope study of liver cell in carbon tetrachloride intoxication, significance of "opaque area"

この論文をさがす

抄録

<p>Electron microscope study on the rat liver cells of carbon tetrachloride poisoning has been reported. Observations have been made on the osmic fixed tissue sections obtained from the liver at early stages of poisoning, 5 to 22 hours after carbon tetrachloride oral administration, 0.25ml. per 100g. body weight. Special attention is paid on the appearance of electron dense area, opaque area, in cytoplasm, which is compased of fibrous components, probably originated from endoplasmic reticulum. This will be an important sign of cell degeneration. Toluidine blue, PAS and methyl green-pyronin stainings of the thicker sections from the same samples as used for electron microscopy revealed that the opaque area is stained by toluidine blue and pyronin but not by PAS. The opaque areas appear already five hours after the carbon tetrachloride administration and show some continuity with elongated filaments of endoplasmic reticulum. At an advanced stage of paisoning the opaque area increases in its number and size, but some of them are shrunk as a mass, being separated from the surrounding cytoplasm with scanty area. Often they form denser masses in the center and loak like the lipid deposition. The picture suggests formation of lipid droplets in the case of fatty degeneration of the liver cell.</p>

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ