Electrical classification of single red blood cell deformability in high-shear microchannel flows

• 巽, 和也

• Tatsumi, Kazuya

• Doi, Tatsuki

• Nakabe, Kazuyoshi

抄録

A sensor that can efficiently and sequentially measure the deformability of individual red blood cell (RBC) flowing along a microchannel is described. Counter-electrode-type microsensors are attached to the channel bottom wall, and as RBCs pass between the electrodes, the time series of the electric resistance is measured. An RBC is deformed by the high shear flow to a degree dependent upon its elastic modulus. Hence, the profile of the resistance, which is unique to the shape of the RBC, can be analyzed to obtain the deformability of each cell. First, theoretical and experimental analyses were conducted to identify the specific AC frequency at which the effect of the electric double layer formed on the electrode surface is minimized. Measurements were then conducted upon samples of normal human RBCs and glutaraldehyde-treated (rigidified) RBCs to evaluate the feasibility of the present method. In addition, simultaneous visualization of RBC deformation was performed using a high-speed camera. Normal RBCs were observed to have a degree of deformation index (DI) of around 0.57, whereas the rigidified RBCs was DI = 0 in the microchannel. The experimental measurements showed a strong correlation between the half-width of the maximum of the resistance distribution and the DI of the RBC.

収録刊行物

• International Journal of Heat and Fluid Flow

  International Journal of Heat and Fluid Flow 31 (6), 985-995, 2010-12

  Elsevier Inc.

キーワード

• Red blood cell
• Deformability
• Microchannel
• Electric sensor
• Laminar flow
• Medical engineering

詳細情報

• CRID: 1050001202178173184
• NII論文ID: 120002661527
• NII書誌ID: AA00234073
• ISSN: 0142727X
• HANDLE: 2433/131864
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles