Production of Homozygous S1 Seedlings for S Gene in 'Hirado Buntan' Pummelo (Citrus grandis Osbeck) and Determination of the S Alleles (S_9 and S_10) by Pollination with the S_1 Seedlings to Citrus Cultivars

• Kim Jung-Hee

  Laboratory of Horticultural Science, Division of Agricultural Plant Science, Department of Plant Resources, Graduate school of Bioresource and Bioenvironmental Sciences, Kyushu University

• 森 智代

  九州大学大学院生物資源環境科学府植物資源科学専攻農業植物科学コース園芸学分野

• 若菜 章

• Ngo Binh Xuan

  Faculty of Biotechnology and Food Technology, Thai Nguyen University of Agriculture and Forestry | Institute of Life Science, Thai Nguyen University

• Masuda Jun'ichiro

• 酒井 かおり

  九州大学大学院農学研究院環境農学部門農業環境科学講座農業生産生態学分野

• 梶原 康平

  九州大学大学院農学研究院環境農学部門農業環境科学講座農業生産生態学分野

抄録

Gametophytic self-incompatibility (SI) in Citrus is known in pummelo, mandarin and many hybrid cultivars with pummelo in their pedigrees. What seems to be lacking, however, is determination of allelic variation in SI gene (S) and the genotypes of self?incompatible and semi self-incompatible cultivars. In previous studies, S genotypes of several Citrus cultivars were estimated on the basis of segregation distortion for Got-3 gene in F1 progenies between these cultivars. In this study, S1 seedlings were produced from self? pollinated ‘Hirado Buntan’ pummelo, and homozygous S1 seedlings for the S gene were determined by the pollination with ‘Hirado Buntan’. Then, the homozygous S1 seedlings were pollinated with the homozygous S1 seedling (SaSa) each other and classified them into two genotype groups of SaSa and SbSb. The ten cultivars, whose S genotypes were predicted on the basis of joint segregation analysis with Got-3 gene in their progenies, were pollinated with the homozygous S1 seedlings (SaSa or SbSb). Pollen tube behavior in the style of the ten Citrus cultivars after pollination with the Sa or Sb pollen led to the conclusion that ‘Hirado Buntan’ has S9 and S10 alleles. Furthermore, pollen tube arrest in the style of self-compatible ‘Kabusu’ sour orange pollinated with S9 pollen suggested that the genotype of ‘Kabusu’ is SfS9. Inscrutably, neither S9 nor S10 alleles were found in a group belonging to the Hirado pummelo line, which started from seeds sown by the 11th feudal load Terasu Matsura of the domain of Hirado. This fact supported the two origins of Hirado pummelo plants described in the literature, and indicated the association of multiple seed and/or pollen parents with different S alleles to the original seed production.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 55 (2), 239-245, 2010-10-29

  九州大学大学院農学研究院

キーワード

• Hirado Buntan
• Pummelo
• S_9
• S_10

詳細情報

• CRID: 1390009224684695936
• NII論文ID: 120002697626
• NII書誌ID: AA00247166
• DOI: 10.5109/18836
• HANDLE: 2324/18836
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles